Job title:

Applications are invited for a Postdoctoral Research Associate (PDRA) position to join a collaborative research program with the aim of gaining an understanding of the mechanisms and role of synaptic dysfunction and neurotransmission impairment in amyotrophic lateral sclerosis and frontotemporal dementia (ALS/FTD).

You will work within a team of two Postdoctoral Research Assistants and two Senior Research Technicians under a 5-year MRC-funded collaborative research programme. The program will include electrophysiological and microscopic-based interrogation of synaptic function in vitro in human induced pluripotent stem cell (iPSC) models of ALS/FTD and primary neuronal cultures. Furthermore, this work will investigate fundamental mechanisms using high-resolution genetic and biochemical perturbations combined with state-of-the-art proteomics and determine the role of synaptic dysfunction as a driver of disease using Drosophila models.

In this post, you will lead the coordinated maintenance of iPSCs and the generation of iPSC-derived neuronal cultures in which synaptic function will be examined using state-of-the-art electrophysiological and microscopic assays. You will contribute to all other aspects of the project, including investigations into the underlying mechanisms. Under the supervision of principal investigators, you will be expected to design, perform and interpret experiments, compile reports and publications, present at laboratory groups and scientific meetings, as well as coordinate and direct the work of technicians supporting the project.

The ideal candidate will be a hands-on, well-organized and dedicated individual with a proven track record in iPSC-based human disease modeling, and ideally with experience in iPSC-derived neuronal cultures. Previous experience in characterizing neuronal function (e.g., electrophysiology), primary neuronal cultures, and lentiviral and AAV vectors would be extremely beneficial. Proficiency in molecular cloning, PCR, biochemistry (Western, IP) and fluorescence microscopy will also be valuable for this role. Candidates must also demonstrate a history of science communication (e.g. presentations, outreach activities, teaching/supervision).

This participation is fixed-term for 3 years with the possibility of a 1-year extension and is available now.

Please note that while the role is at post-doctoral research level and requires candidates to hold a PhD/PhD (or equivalent), we may consider candidates who are close to completing a PhD/PhD qualification, in which case the initial appointment will be in Year 6 (increased to Year 7 on completion of the PhD/PhD qualification).

Candidates are strongly encouraged to contact Professor Kurt de Vos (k.de_vos@sheffield.ac.uk) and Dr. Matthew Livesey (mrlivesey@sheffield.ac.uk) to discuss the project before submitting the application.

We build teams of people of different heritages and lifestyles from around the world, whose talents and contributions complement each other to achieve maximum impact. We believe that diversity in all its forms achieves greater impact through research, teaching and student experience.